International Journal of Chemistry Research 2023-08-29T12:49:47+00:00 Editor [email protected] Open Journal Systems <p>International Journal of Chemistry Research (IJCR) is a peer-reviewed, open access journal, published by Innovare Academic Sciences Pvt Ltd. It publishes Research Articles, Short communications and Review Articles in all areas of chemistry.</p> <p>Authors can submit complete unpublished and original works, which are not under review in any other journals. The scopes of the journal include, applied chemistry, inorganic chemistry, analytical chemistry, organic chemistry, physical chemistry, structural chemistry, polymer chemistry, nuclear chemistry, chemical engineering, environmental chemistry, medicinal chemistry and pharmaceutical chemistry.</p> <p><strong>Indexing and Abstracting-</strong>&nbsp;OAI, LOCKKS, Google Scholar, OCLC (World Digital Collection Gateway), UIUC</p> PHYTOCHEMICAL CONSTITUENTS AND ANTIOXIDANT ACTIVITY OF PERSEA AMERICANA LEAVE 2023-04-20T09:14:31+00:00 AMUDA MUTIU OLASUNKANMI [email protected] ODERINLO OGUNYEMI [email protected] <p><strong>Objective</strong>: <em>Persea americana</em> is a popular plant that is well known for its fruit, which is highly consumed due to its nutritional and medicinal values. The rich secondary metabolites content of <em>P. americana</em> creates an opportunity to utilize it as a functional food or standardized herbal preparations. The quality of a medicinal plant is determined by the active substances produced by the plant. Therefore, the purpose of the present study is to determine the secondary metabolites present in <em>P. americana</em> grown in uncultivated farmland in Ogbomoso, Oyo State Nigeria.</p> <p><strong>Methods</strong>: The dried pulvurised leaves of <em>P. americana</em> from uncultivated farmland in Ogbomoso, Oyo State Nigeria were extracted with methanol by the maceration method and partitioned successively with solvents of increasing polarity. The extracts obtained were subjected to phytochemical screening and the total phenolics were determined spectrometrically using the folin–ciocalteu method.</p> <p><strong>Results</strong>: The phytochemical screening showed that <em>P. americana</em> is rich in various secondary metabolites such as flavonoids, saponins, tannins, and alkaloids. The total phenolic content gave 500µg/g gallic acid equivalent. The total flavonoid content obtained was 250µg/g using quercetin as standard. The anti-oxidant assay of the crude extract showed a high activity of 89.05% at a concentration of 0.05g/ml.</p> <p><strong>Conclusion</strong>: Total phenolic content of the plant is likely to be a key for determining the free radical scavenging and ROS reducing ability of the plant.</p> 2023-07-01T00:00:00+00:00 Copyright (c) 2023 AMUDA MUTIU OLASUNKANMI, ODERINLO OGUNYEMI SPECTROPHOTOMETRIC FINGERPRINTING AND CHEMICAL DETERMINATION OF STREPTOMYCIN, AMIKACIN, NEOMYCIN, AND GENTAMYCIN SULPHATE BY CONDENSING WITH NINHYDRIN REAGENT 2023-05-03T05:41:55+00:00 EDEBI N. VAIKOSEN [email protected] SAMUEL J. BUNU [email protected] EBIERE DODE [email protected] RUTH B. EFIDI [email protected] <p><strong>Objective: </strong>The study aimed to develop a simple, efficient, inexpensive, rapid, and reproducible spectrophotometric analytical technique for aminoglycosides analysis by condensing with ninhydrin reagent.</p> <p><strong>Methods</strong>: At a pH of 8.0, different aminoglycosides, including amikacin, gentamycin, neomycin, and streptomycin, were deaminated by the ninhydrin reagent. The working standard solution for each component was accurately placed into a series of 10 ml calibrated volumetric flasks, then 1 ml of the ninhydrin reagent was added and heated in a water bath for 15 min. The wavelength(s) of maximum absorption(s) was recorded after scanning the resultant purple complex from 350 nm to 930 nm. The final working concentrations ranged between 1600 and 2000 ug/ml.</p> <p><strong>Results</strong>: The average weight of a neomycin sulphate tablet was 0.747g, with a standard deviation of 0.667%. After complexing with ninhydrin reagent at pH 6.0 for 10 to 15 min, all aminoglycosides developed purple coloration that lasted beyond 24 h, compared to the initial white, pale yellow, and colorless appearance of streptomycin, amikacin, neomycin, and gentamycin, respectively. The scanned spectra of the purple complex of ninhydrin formed after reaction with aminoglycosides in the visible region (350-900 nm) were similar, indicating the presence of a common parent structural moiety. At 550 nm, 650 nm, 750 nm, and 850 nm, distinct absorptions were observed. Amikacin, Streptomycin, and Gentamicin had the highest absorbance between 800 and 900 nm. After the reaction with ninhydrin, three distinct absorbances were observed in the Neomycin spectrum: between 380 and 400 nm, 580 and 600 nm, and around 800 nm. The comparative spectra for the four aminoglycosides and ninhydrin reagent with blank show a unique feature for the compounds. The coefficients of regression were 0.996 and 0.995, respectively.</p> <p><strong>Conclusion</strong>: The proposed methods for analyzing streptomycin in streptomycin injections were successful. The percentage purity ranged from 017–110 % at 650 and 850 nm, which corresponds to the British Pharmacopoeia (BP) limit that streptomycin sulphate tablets should be between 97.00 and 110%.</p> 2023-07-01T00:00:00+00:00 Copyright (c) 2023 EDEBI N. VAIKOSEN, SAMUEL J. BUNU, EBIERE DODE, RUTH B. EFIDI